Methods In Molecular Medicine Clinical Applications Of Capillary Electrophoresis Hardcover only $ 40.00 - ZoneOffer
Methods In Molecular Medicine Clinical Applications Of Capillary Electrophoresis Hardcover

Methods In Molecular Medicine Clinical Applications Of Capillary Electrophoresis Hardcover

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The term electrophoresis was first used by michaelis in 1909 to
Scribe the migration of colloids in an electric field. The first practical elect- phoresis method was described by tiselius in 1937. He used a u-tube filled with buffer layered on top of sample migration could be monitored using schlieren optics. In zone electrophoresis the u-tube was replaced by paper a support material employed simply to prevent or minimize diffusion of ions so that ions applied in a narrow strip to the paper will separate and remain as relatively discrete zones. Paper was superceded by a variety of other media
Cluding cellulose acetate hydrolyzed starch starch gel agarose and polyacry- mide. The latter in addition to being a support medium has size-sieving properties. From the basic zone electrophoresis other means of separation have been dev- oped. These include isoelectric focusing isotachophoresis density gradient el- trophoresis and various forms of immunoelectrophoresis. In some ways capillary electrophoresis ce has gone full circle back to the original method of tiselius. In its simplest form separations occur in a buffer solution within a glass fused silica tube and detection occurs as sample moves past an optical window. Ce has rapidly developed into a technique that rivals hplc in its versatility. All the classical electrophoretic separations-zone ief and isotachophoresis-have their counterparts in ce. Excitingly so and
Thoritatively treated in clinical applications of capillar...
Brand: Humana Press
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